Abstract
Background The state of Espirito Santo has in the fruit growing, one of its main economical activities. One of them, the pineapple growth, represents a good economic potential to increase income and job opportunities in the state. However, diseases such as fusariosis and mealybug wilt of pineapple are responsible for the yield losses of up to 80% [1]. The mealybug wilt of pineapple is caused by the complex Pineapple mealybug wilt-associated virus (PMWaV-1, PMWaV-2, PMWaV-3 and PMWaV-5), a single strand RNA [2], while fusariosis on pineapple is caused by the fungus Fusarium guttiforme [3]. This study aimed to develop methodologies for detection of these etiological agents in the pineapple plant.
Highlights
The state of Espirito Santo has in the fruit growing, one of its main economical activities
For the analyzes, healthy and diseased pineapple plant were collected at the Experimental Farm of Incaper in the city of Sooretama, in the state of Espirito Santo
The samples were submitted to a superficial disinfection process, followed by nucleic acid extraction protocols with Trizol® and SDS buffer [4]
Summary
Molecular diagnosis of Fusarium guttiforme and Pineapple mealybug wilt-associated virus. Lorena Carnielli1*, Walkíria Andrade Amorim, Aline Vaz, Patricia Machado Bueno Fernandes, José Aires Ventura. From 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC) Florianópolis, Brazil. From 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC) Florianópolis, Brazil. 10-14 November 2013
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