MOLECULAR DIAGNOSIS OF DIFFERENT ISOLATES OF SEED GALL NEMATODE (ANGUINA TRITICI) IN IRAQ AND IT'S CONTROL

Abstract

This study was conducted on seed galls disease, it,s the most important and serious diseases on wheat and barley in Iraq, caused by Anguina tritici. Wheat seed galls were geathering from different areas of Iraq ( Tal Afar, Qayyarah, Hawija, AlDur, Al-Alam, Mosul, Tilkayf, Baghdad, Erbil ) as well as barley seed galls from Ba'shiqah, polymerase chain reaction (PCR) by specific primers was used to confirmed morphological dignostic of wheat seed galls nematodes, while the barley seed gall nematode had been identified for the first time in iraq . Random Amplified polymorphic DNA (RAPD) showed up genitic various in wheat and barley seed galls isolatese . There were a 99% similarity in nucleatied sequence of ITS gene for isolates with nucleated cintre of biotechnology information . Genetic amutation had been in all isolates and Talafar and Erbil 2 isolates were the highest with 240 nucleotide number replaced the G instide of A