Abstract
The aim of the present study was to diagnose caseous lymphadenitis in sheep by PCR assay . A total of 1257 sheep from different flocks in Dakahlia governorate were clinically examined during the period of January 2014 to February 2015. sheep were classified according to the status of infection in the flocks to infected , incontact and free ones. 37 needle biopsy samples were randomly collected from 26 diseased, 7 incontact and 4 free examined sheep for PCR assay. rpoB gene was detected in 37 samples (100%) while PLD gene was in 18 samples (48.64%), from which 16 from diseased sheep and two from incontact ones, while not detected in free sheep. The PCR assay was rapid, specific and significant technique as bacterial culture in detecting corynbacterium pseudotuberculosis in needle biopsy samples from sheep
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