Abstract
Molecular diagnosis of benzimidazole resistance in Haemonchus contortus in sheep from different geographic regions of North India
Highlights
Benzimidazole group of anthelmintics are widely used for control of Haemonchosis in sheep due to their high therapeutic index and absence of toxic residues in milk and meat [1]
Species was confirmed as H. contortus by polymerase chain reaction (PCR)-Restriction Fragment length polymorphism (RFLP) which shows three fragments at 440 bp, 190 bp, and 150 bp
Due to indiscriminate use of Benzimidazole group of drugs in organized and private farms, resistance to this group of drugs is increasing at the field level
Summary
Benzimidazole group of anthelmintics are widely used for control of Haemonchosis in sheep due to their high therapeutic index and absence of toxic residues in milk and meat [1]. Benzimidazole (BZ) exhibit the anthelmintic activity by binding to β-tubulin which interferes with polymerization of the microtubule. The diagnosis of BZ resistance in a worm population of Trichostrongyle parasites was based until now on the estimation in vitro of the lethal dose 50 (LD50) on eggs (Egg hatch assay), on larvae (Larval development assay), or in vivo of the egg reduction in faecal sample after BZ treatment (FECRT) [3, 4, 5]. These methods have proved their efficacy but can only detect BZ resistant worm population when more than 25% of the parasites are BZ resistant [6].
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