Molecular Diagnosis and Evolutionary Relationship Analysis Of Plant Parasitic Tea Garden Nematodes From Different Tea Estates In Sylhet Region of Bangladesh

A Iqbal,Mmh Sohag,Msh Chowdhury,Mab Siddique,K Islam,Ta Asa

doi:10.3329/jbs.v29i0.54826

A Iqbal, Mmh Sohag + Show 4 more

Open Access

https://doi.org/10.3329/jbs.v29i0.54826

Copy DOI

Abstract

Nematodes from plant-parasitic sources are ever-present and incidental to plant growth as well as crop production. The damage of tea gardens caused by nematode is often non-specific and easily confused with symptoms. The present study determined the parasitic and non-parasitic nematodes population in different tea gardens of the Sylhet region by their morphological and partial molecular characterization. Out of 13 tea gardens, it was observed that BTRI, Karimpur, Mathiura, and Tarapur tea garden has the highest number of parasitic and non-parasitic nematodes. After PCR amplification, DNA bands with desired amplicon size were detected by gel electrophoresis. Among thirteen soil samples, nematodes from Malnichara, Karimpur, BTRI, Mathiura , and Finlay had partially confirmed the presence of rootknot nematode (Meloidogyne spp.), root-lesion nematode (Pratylenchus brachyurus), burrowing nematode (Radopholus similis), reniform nematode (Rotylenchulus reniformis) and lance nematode (Hoplolaimus columbus) consequently based on approximately base pair of 1.7, 1.1 and 0.52 kb (different Meloidogyne spp.) 0.52, 0.52, 0.25 and 2.3 kb of specific genes. From evolutionary analysis, it might be said that Meloidogyne species are strongly related with each other making clusters except Meloidogyne natalie where this one is closely related with Hoplolaimus columbus in their evolutionary relationship as remaining others (Rotylenchulus reniformis, Radopholus similis, Pratylenchus brachyurus) are in different clusters in the same clade and this result could be confirmed after sequencing. J. Bio-Sci. 29(1): 101-109, 2021 (June)

Highlights

Tea, Camellia sinensis, is a principal perennial cash crop in Bangladesh that has a great impact on agricultural export and domestic demand for sustaining the economy of Bangladesh
Based on morphological characteristics of nematodes isolated from soil samples, plant-parasitic nematodes were identified in 9 of 13 soil samples from different tea gardens, with densities ranging from 50 to 200 individuals per 5 ml of nematode solution extracted from 200 g of soil (Table 2)
Molecular diagnosis is important for the confirmation of plant-parasitic nematode species identification and analysis of their phylogenetic relationships with available sequences in databases

Summary

Introduction

Camellia sinensis, is a principal perennial cash crop in Bangladesh that has a great impact on agricultural export and domestic demand for sustaining the economy of Bangladesh. Tea is a popular nonalcoholic drink made from the leaves of an evergreen shrub or tree of Camellia sinensis belonging to the family Theaceae over the world. Tea is cultivated as a monoculture over large adjacent areas during the last 165 years and generated a strong tea ecosystem for various insects, mites, and nematodes in Bangladesh. Chittagong, and Phanchagarh district is the main ecological zone for the cultivation of tea in Bangladesh. The spreading and persistency of these insects, mites, and nematodes are responsible for the performance of shade trees, ancillary crops forests, a uniformity of cultural practices such as sequential pruning cycles, weekly plucking rounds, weeding, mulching, and so on.

Methods

Results

Conclusion