Abstract
Molecular Detection of<i>Ehrlichia chaffeensis</i>in<i>Amblyomma parvum</i>Ticks, Argentina
Highlights
In Central and South America, human cases of ehrlichiosis with compatible serologic evidence have been reported in Venezuela, Brazil, Mexico, and Chile, the bacterium has not been isolated (3)
A sample of 70 A. parvum and 1 A. tigrinum ticks collected on domestic ruminants and canids were subjected to PCR and reverse line blot hybridization by using the TBD-RLB membrane (Isogen Life Science, Maarssen, the Netherlands) (6) to look for Anaplasma and Ehrlichia spp
PCR products were analyzed by reverse line blot hybridization, and 11.3% (95% confidence interval [CI] = 4.9–21.0) showed a positive signal to the specific E. chaffeensis probe: 8 A. parvum ticks collected from a dog (n = 1), a fox (Lycalopex gymnocercus, n = 1), goats (n = 2), and cattle (n = 4)
Summary
In Central and South America, human cases of ehrlichiosis with compatible serologic evidence have been reported in Venezuela, Brazil, Mexico, and Chile, the bacterium has not been isolated (3). PCR products were analyzed by reverse line blot hybridization , and 11.3% (95% confidence interval [CI] = 4.9–21.0) showed a positive signal to the specific E. chaffeensis probe: 8 A. parvum ticks collected from a dog (n = 1), a fox (Lycalopex gymnocercus, n = 1), goats (n = 2), and cattle (n = 4). E. chaffeensis was detected in A. parvum ticks only: 5 from humans (6.2%; 95% CI 2.1–14.0; Figure, panel A) and 3 from host-seeking ticks (16.7%; 95% CI 3.6–41.4).
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