Abstract
The current study was aimed to genotypically detection of genes encode outer membrane proteins related with human serum resistance. A total of 33 isolates were identified as Klebsiella pneumonia according to the manual cultural characteristics and biochemical tests and then confirmed by Vitek -2 system. The number of isolates according to site of infection were 14 (42.4%) isolates from wound infection, 15(45.4%) isolates from urine, 1(3.03%) isolates from blood, 1(3.03%) isolates from vagina,1(3.03%) from ear swab and finally 1(3.03%) isolates from stool. The present study demonstrated a positive relationship between the ability of bacterial isolates to antibiotics resistance and the occurrence of some virulence genes. The molecular detection of some virulence genes (htrA, rmpA, mrkA and iss) was done by conventional PCR technique. It was found that ,high occurrence of htrA gene was observed among Klebsiella pneumonia isolates which was found in 26(78% ) of isolates at 1071bp , then 17(51%) isolate were positive for rmpA gene at 967 bp,whereas mrkA gene was recorded in 5(15.15%) isolates at 862 bp , and only 4(12.12%) isolates gave positive result for iss gene at 450bp.
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