Abstract
Molecular Detection of vapA and vapB Genes in Rhodococcus equi Isolated from Human and Horses by Multiplex PCR in Baghdad
Highlights
Rhodococcus equi (R. equi) is a facultative intracellular, Gram positive, non-motile bacterium widely spread in the soil and the surrounding of the animals keeping like horse stables (Radositis et al, 2007; Le et al, 2015)
Rhodococcus equi Isolation The suspected colonies appeared as dark grey to black, sometime as dark brown in colour and mucoid and moist in consistency on novobiocin–actidione cycloheximide–potassium tellurite (NANAT) medium (Figure 1), and appeared as irregularly round, smooth, semitransparent, glistening, coalescing mucoid and salmon- pink in colour on blood agar with age (Figure 2)
Results of multiplex PCR assays of 11 isolates from human and horses samples showed that only 57.14% (4/ 7) of the R. equi isolates from horses samples were vapA positive, all the 4 R. equi isolates from human sputum were negative for both vapA and vapB gene (Figure 4)
Summary
Rhodococcus equi (R. equi) is a facultative intracellular, Gram positive, non-motile bacterium widely spread in the soil and the surrounding of the animals keeping like horse stables (Radositis et al, 2007; Le et al, 2015). It is an opportunistic bacterium, causing rhodococcosis, a zoonotic problem that can be confused with tuberculosis (Silva et al, 2010). Infections with this pathogen has been detected in a number of animal species like cattle, pigs, deer, dogs, goats, cats, sheep, and wild animals (Prescott, 1991; Sakai et al, 2012; Cohen et al, 2014). Since the isolation of this pathogenic bacterium, the number of human cases increased (Weinstock and Brown, 2002).
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