MOLECULAR DETECTION OF UREC, HPMA, RSBA AND MRPA GENES OF PROTEUS MIRABILIS URINARY TRACT INFECTION IN PATIENT WITH RHEUMATOID ARTHRITIS

Al-Hamdani & Al-Hashimy

doi:10.36103/ijas.v51ispecial.902

Abstract

This study was aimed to detect of four important virulence genes (UreC, HpmA, rsbA and mrpA) from Proteus mirabilis bacteria isolated and do the important four blood test (ACPA, RF, ESR, CRP) for patient with urinary tract infection and rheumatoid arthritis in Baghdad/Iraq. Fifty (50) clinical urine samples were collected and cultured on blood agar and macckongy agar. Ten of the samples were identified as P.mirabilis and the automated system VITEK 2 used to complete the identification. Molecular methods using PCR technique targeting UreC, HpmA, rsbA and mrpA genes, using PCR technique and the results were UreC found in 90% of the samples, HpmA 90%, rsbA 100% and mrpA 80%. The P. mirabilis samples were also characterized for antibiotic resistance and the result showed that the majority of samples were show high resistant to Cefotaxime 90%, while the highest susceptibility was against Impenem antibiotic 70%.

Highlights

Rheumatoid arthritis RA is one of the more common autoimmune diseases, affecting approximately 1% of the population worldwide
Different immunological and microbiological studies results support that there could be a link between urinary tract infections (UTI) and RA, this has mainly caused by P.mirabilis bacterium (10)
P. mirabilis bacteria have a significant role in causing urinary tract infection which can be defined as the inflammatory response of urothelium to bacterial invasion, which is usually associated with bacteriuria and pyuria

Summary

Introduction

Rheumatoid arthritis RA is one of the more common autoimmune diseases, affecting approximately 1% of the population worldwide. Proteus mirabilis is a Gram-negative, facultative anaerobic rod shaped bacterium It may found as part of the normal flora in intestine. This organism is not usually a pathogen, but does become a problematic when it comes into contact with urea in the urinary tract, So that, the infection can spread to the other parts of the body. This bacterium is well-known for its urease enzyme production and distinctive ability to differentiate into elongated swarm cells. This study was aimed to detect of four important virulence genes (UreC, HpmA, rsbA and mrpA) from Proteus mirabilis

Objectives

Methods

Results