Abstract
Molecular detection of tick-borne pathogens in ticks from Uganda
Highlights
Tick-borne diseases are among major constraints to livestock production throughout Sub-SaharanAfrica
Rickettsia africae transmitted by Amblyoma variagetum, is the aetiologic agent of African tick-bite fever - an emerging zoonosis in rural sub-Saharan Africa and the Caribbean
Rickettsia conorii transmitted by Rhipicephalus appendiculatus causes tick typhus in humans
Summary
Tick-borne diseases are among major constraints to livestock production throughout Sub-Saharan. Cephalus (Boophilus) decoloratus transmits Borrelia theileri, the cause of spirochaetosis in cattle, sheep, goats and horses. Rickettsia africae transmitted by Amblyoma variagetum, is the aetiologic agent of African tick-bite fever - an emerging zoonosis in rural sub-Saharan Africa and the Caribbean. Rickettsia conorii transmitted by Rhipicephalus appendiculatus causes tick typhus in humans. Ticks were surface-sterilized by a 1000μl DDW wash, two washes in a 75% ethanol solution, followed by a. The sample was homogenized twice by Micro Smash MS-100R (TOMY, Tokyo, Japan) in the presence of stainless steel beads (1.0 mm in diameter) for 2 min at 2,500 rpm, followed by DNA extraction with. The sample was shaken vigorously and left at 4°C for 2 hours to sediment the DNA followed by two washes with 75% ethanol. The solution was neutralised by adding 2 μl of 1 M
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