Molecular Detection of the Gene (Glutathione S-Transferase Zeta Class-Like) Responsible for Stress Tolerance and Studying Its Genetic Expression in a Number of Snake Melon Cultivars

Abstract

The experiment was conducted in the winter agricultural season 2020 in the greenhouses of the Al-Fares Company in Al-Zubair district, with the aim of investigating one of the important genes responsible for tolerating Armenian cucumber for stress conditions, which is the gene (glutathione S-transferase zeta class-like) in 21 Armenian cucumber cultivars. Isolation of RNA from leaves after 100 days of cultivation at the saline level 5 dSm−1, as well as a control treatment of 1 dSm−1and studying their gene expression using RT-qPCR (Real Time-quantitative Polymerase Chain Reaction) technique. The results showed the excelled of the cultivars under the influence of salt stress, Egypt, Babylon, Mosul (Carmelis), Kirkuk, Diyala and Karbala, as they gave the highest expression of their gene expression (25.12, 21.87, 19.04, 21.87, 19.04 and 20.40), respectively, so they can be considered salt-tolerant cultivars. This is because the expression values for the gene (glutathione S-transferase zeta class-like) were high compared to the rest of the cultivars. As for the cultivars whose sensitivity to salinity was confirmed by the experiment, they are Iranian (Ghani), Italian, Baghdad and Amara. These cultivars gave the lowest gene expression values (2.73, 4.46, 4.14 and 3.49), respectively.