Molecular detection of some virulence genes of Proteus mirabilis in rheumatoid Ar-thritis patient’s urine and other clinical samples

Abstract

This study was aimed to detecting three different virulence genes (UreC, HmpA and ZapA ) for Proteus mirabilis isolated from different clinical samples ( urine from UTI non-Rheumatoid arthritis patients, urine from UTI Rheumatoid arthritis patients, wound swaps , chronic supportive otitis media(CSOM) and cerebrospinal fluid aspirate(CSF). Three hundred (300) different clinical samples were collected and cultured on blood agar and macConky agar. 40 of samples were identified as P. mirabilis , biochemical test and VITEK 2 system used to complete the identification. Phenotypic detection appeared the present 100% of urease enzyme (on urea agar) hemolysin enzyme ( on blood agar), swarming phenomena (on agar plate ) while protease enzyme detection (on skim milk agar) present in 54% of isolates. Molecular results by PCR present UreC, HmpA and ZapA genes in 100 % in 40 isolates. Antibiotic Susceptibility test of Proteus mirabilis based on Kirby-Bauer disk diffusion method on Muller-Hinton agar to (14) different antimicrobial agents appeared 100% resistance to Aminoxicillin /clav, Cefotaxime and Erythromycin while the lowest resistance was against Impenem, Ciprofloxacin, Meropenem and Gentamicin (12.5%, 15 %, 20% and 47.5%), respectively.