Molecular Detection of Pseudomonas Aeruginosa Isolated From Chicken Cans in the Markets of Al-Muthanna Province

Abstract

Food safety is very necessary for the general health of the consumer and protection from disease, as well asthe importance of economic sustain ability of the agricultural food sector. The consumer wants to make surethat the food is safe and that it is not contaminated with bacteria. Therefore, these tests must be performedto detect bacteria and ensure that food safety.120 samples was collected from frozen chicken cans from the markets of Al-Muthanna province, using thesterile plastic bags and after isolation the results showed that (55) sample contain bacteria Pseudomonasaeruginosa from the total samples above.The results of bacterial culture were positive in 45 samples whereas the results of direct molecularidentification were positive in 55 samples.Selective media used for bacteria identification such as MacConkey Agar, Methyle red which has been giventhe proportion of isolation (15% and 85% respectively) also some isolates showed their ability to liquefygelatin (65%) and fermented sugar lactose (100%) . however biochemical testing also used for all bacterialisolates for confirm diagnoses.Polymerase chain reaction technique used for detection bacteria by specific primers sequence the resultappear 45% of total sample contamination by P. aeruginosa .The genome of P. aeruginosa is relatively large so encodes a large proportion of regulatory enzymesimportant for metabolism and efflux of organic compounds. This enhanced coding capability genome allowsfor great metabolic versatility and high adaptability to environmental changes,According our result we recommend food factories to use sterilized materials more accurately and effectivelyto store frozen chicken cans.