Abstract
BackgroundPorcine astrovirus (PoAstV) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (PoAstV1–PoAstV5) within the genus Mamastrovirus. However, our knowledge of the diversity and epidemiology of PoAstV in China is limited.ResultsIn this study, fecal samples from 21/120 (17.5 %) domestic pigs, including 18/100 (18 %) diarrheic and 3/20 (15 %) healthy pigs, and from 1/9 (11.1 %) healthy wild boars tested in Sichuan Province were positive for PoAstV on reverse transcription–PCR. Of the 22 positive samples, 13.6 % were positive for PoAstV only, whereas 40.9 % also contained Porcine epidemic diarrhea virus (PEDV), 22.7 % also contained porcine group A rotavirus (PRoVA), and 22.7 % also contained PEDV and PRoVA. A phylogenetic analysis of the RdRp gene revealed genetic heterogeneity among the PoAstV sequences and two lineages were detected in this study, with PoAstV-2 predominant. PoAstV-5 was detected in wild boars for the first time.ConclusionsPoAstV infections exist in Sichuan Province regardless of the disease status in the pig population, either alone or in combination with other enteric viruses, and may be associated with diarrhea.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-015-0462-6) contains supplementary material, which is available to authorized users.
Highlights
Porcine astrovirus (PoAstV) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (PoAstV1–PoAstV5) within the genus Mamastrovirus
PAstV-5 has been identified in domestic pigs, with or without diarrhea, in five countries, including the USA [3, 7], Sweden [4], Canada [6], China [8], and Croatia [2, 9], whereas no study has reported PAstV-5 in wild boars
Nine fecal samples from clinically healthy wild boars were collected from wildlife areas located in the northwest of Sichuan Province in December 2014
Summary
Porcine astrovirus (PoAstV) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (PoAstV1–PoAstV5) within the genus Mamastrovirus. Porcine astrovirus (PoAstV) was first reported in 1980 in fecal samples from diarrheal pigs, and PoAstV was A number of PoAstV isolates have been detected in several countries and characterized, and at least five distinct PoAstV lineages have been identified since PAstV-5 was first identified in fecal samples from slaughtered pigs in Canada in 2009 [6].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
