Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

Rocío Requena-Castro,Rosa Eminé Rodríguez-Reyna,Prisco Palma-Nicolás,Virgilio Bocanegra-García,Miguel Ángel Reyes-López

doi:10.1590/0074-02760160468

Rocío Requena-Castro, Rosa Eminé Rodríguez-Reyna + Show 3 more

Open Access

https://doi.org/10.1590/0074-02760160468

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Abstract

This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21.5% of samples, and the predominant coinfection consisted of DENV-1 and DENV-2. Therefore, continuous epidemiological surveillance of DENV in this area is required to predict future forms of dengue heterologous infections and the effect of this on health care.

Highlights

This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzymelinked immunosorbent assay (ELISA NS1)]
Identifying DENV serotypes is very important because heterologous infections with Dengue Haemorrhagic Fever (DHF) are of great concern (Hammon et al 1960)
The WHO SEARO (2011) indicates that the age groups most affected by dengue are infants, children and adults, our results agreed with Torres-Galicia et al (2014), which found that the incidence of dengue and DHF has increased among young people

Summary

Introduction

This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzymelinked immunosorbent assay (ELISA NS1)]. Identifying DENV serotypes is very important because heterologous infections with Dengue Haemorrhagic Fever (DHF) are of great concern (Hammon et al 1960). These samples were obtained between August and October 2013 (106 men, 137 women) in Matamoros City, Mexico. Out of the 243 suspected dengue samples, 136 (56%) were positive by ELISA NS1, including 76 women and 60 men.

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