Molecular detection of Listeria monocytogenes in raw milk in Kerbala Province

Abstract

Listeria monocytogenes was discovered as a source of contamination during sampling of raw milk and its products from local cows at dairy farms, markets, and shipping points. The goal of the current study was to define the accuracy of listeria monocytogenes in raw milk contamination. This study was conducted by randomly collecting samples from various areas in the Karbala province and its surroundings. From January to March 2022, 250 samples of milk and dairy products (yogurt, cheese, and butter) were randomly selected, processed in accordance with Listeria monocytogenes standard procedures, and sent to a lab from individual cows for analysis. Some biochemical experiments were conducted to ascertain the physical and biochemical characteristics of milk and handled in accordance with Listeria monocytogenes standard procedures before being delivered from the individual cows for analysis to the lab, the study includes the isolation and molecular detection of Listeria monocytogenes from animal sources by employing selective media for this bacteria (oxford agar) for growing, as well as the molecular analysis of L. monocytogenes performed by conventional PCR technique using particular 4 primers (Hyl-1-f, Hyl-2-R, List-F, List-R).