Abstract
Antibiotic resistant Salmonella represent a public health threat worldwide. The current study was delineated to determine Salmonella spp. prevalent in poultry droppings (N: 14 flocks), calves feces (N: 90) and human stool samples (N: 80) from live-stock contacts as well as poultry handler in Beni-Suef governorate, Egypt. Phenotypic and genotypic screening for the presence of antimicrobial resistance was applied. Isolation rates were 57.1%, 5.6% and 2.5% for poultry, calves and humans, respectively. S. Megherafelt. S. Kentucky, S. Amsterdam, S. Nigeria, S. Paratyphi: B2, S. Alamo, S. Madras and S. Haifa were the predominant serovars in poultry flocks under investigation. The predominant serovar from calves’ samples was S. Kentucky while S. Infantis and S. Paratyphi: B2 were detected in human samples. The results of antimicrobial disc diffusion revealed the presence of multiple drug resistance (MDR) in100% of Salmonella isolates. PCR confirmed the existence of class 1 integron and the following gene cassettes in the resistant strains, blaTEM and blaSHV for β-lactams, aadA2 for aminoglycosides (streptomycin), tetA and tetB for tetracycline, sull for Sulfamethoxazole, dfrA for trimethoprim, floR for chloramphenicol and mphA for macrolides.
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