Molecular detection of infectious bronchitis virus with genotyping of broiler chicken flocks in Thi-Qar province, Iraq

Abstract

Infectious bronchitis in poultry is one of the economically important diseases in the poultry industry. study was aimed to diagnose Infectious bronchitis virus (IBV) in broiler chickens using RT-PCR. the current study was completed to diagnose infection in (18) broiler chicken fields in Thi-Qar province. During the period of October 2020 AD to May 2021 AD. samples were collected – swabs were examination results from a total fields, five samples were prepared from each flock, 90 samples were used for real time- PCR test and this result indicated the highest levels of viral RNA titer, 40 samples were positive (44.44%). The highest rates of infected chicks with IB detected in Rifai and Fajr (66.67%). Viral RNA from the (pooled trachea, lung, kidney) with the sample number (A1830242.001) showed positive results with species-specific and IBVvariant-specific RT-PCR, in this study recording the strain Variant2² (Israel02,IS 1494).