Molecular Detection of Human Papilloma Virus Type 16 among Conjunctival Intraepithelial Neoplasia Patients in Khartoum State, Sudan

Ahmed Hala H,Badri Ali M,Aidrous Isra M,Almtry Aya A,Arbab Mohamed H,Ali Sara M

doi:10.23937/2469-567x/1510059

Abstract

Many factors like exposure to UV radiation, climatic conditions, genetic predisposition, immunological state and, more recently, the presence of HPV have been implicated in the genesis of some lesions of the conjunctiva, especially the carcinoma. The study was carried out to detect Human Papilloma Viruses HPV type 16 in conjunctival intraepithelial neoplasia patient in Khartoum state, Sudan during the period from March to July 2019. A total of 50 Formalin-fixed paraffin-embedded Samples were collected from conjunctival intraepithelial neoplasia patients attending the Radiation-Isotopes Centre, Khartoum, Sudan by using polymerase chain reaction. Out of 50 patients 30 (60%) males and 20 (40%) females, with a mean age of 38 years, in range from 15-70 years. The result showed 7 (14%) was positive for HPV-16 and 43 (86%) were negative. Our results show that HPV-16 infection is associated with conjunctival intraepithelial neoplasia, but does not act alone in the development of conjunctival epithelial neoplasia. However, additional studies with a larger number of samples will be necessary.

Highlights

Papillomaviruses are small, non-enveloped, epitheliotropic, double stranded DNA viruses that infect mucosal and cutaneous epithelia in a wide variety of higher vertebrates in a species-specific manner and induce cellular proliferation
Our results show that Human Papilloma Virus Type 16 (HPV-16) infection is associated with conjunctival intraepithelial neoplasia, but does not act alone in the development of conjunctival epithelial neoplasia
HPVs have been identified in association with a variety of benign and malignant epithelial lesions ranging from common cutaneous warts, respiratory papillomatosis, pterygium, and papilloma of the conjunctiva to an expanding epidemic of sexually transmitted anogenital condyloma and cervical cancer [1,2]

Summary

Introduction

Papillomaviruses are small, non-enveloped, epitheliotropic, double stranded DNA viruses that infect mucosal and cutaneous epithelia in a wide variety of higher vertebrates in a species-specific manner and induce cellular proliferation. HPVs have been identified in association with a variety of benign and malignant epithelial lesions ranging from common cutaneous warts, respiratory papillomatosis, pterygium, and papilloma of the conjunctiva to an expanding epidemic of sexually transmitted anogenital condyloma and cervical cancer [1,2]. The conjunctiva is the clear, thin membrane that covers part of the front surface of the eye and the inner surface of the eyelids. It has two segments includes Bulbar conjunctiva, which it’s the portion of the conjunctiva covers the anterior part of the sclera (the “white” of the eye).

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