Molecular Detection of Human Papilloma Virus 31 and 33 in Prostate Carcinoma and Prostate Benign Tissues from a Group of Iraqi Patients

Abstract

Background: Human papillomavirus (HPV) has been connected to the progress of different types of human cancers. Prostate cancer is the second most common cause of cancer in men that might have such relation with Human papillomavirus (HPV). Objective: To assess the rates of occurrence HPV infection in the samples of prostate tissues with carcinoma and benign. Patients and methods: Seventy blocks of formalin-fixed, paraffin- embedded prostate tissues were used in present study; (30) biopsies from prostate carcinoma, (20) biopsies from prostate benign tissues and (20) from apparently normal prostate tissues as a control group. Detection of HPV-31 and 33 was done by ultra-sensitive version of (ISH) whereas immunohistochemistry (IHC) technique was followed to display the cdk2 gene expression. Results: Detection of HPV-31 and 33-CISH reactions in the prostate carcinoma tissues was observed in 17 out of 30 (46.67%), while in those tissues with prostate benign was 35% (7 out of 20). No positive HPV-31 and 33-CISH reactions in apparently normal prostate tissues of control group were revealed. The positive CDK-2-IHC reactions was detected in 46.67% (14 out of 30 cases) of prostate carcinoma and 30% (6 out of 20 cases) of benign prostate.The statistical difference between the rates of CDK-2 in prostate carcinoma and prostate benign tissues was highly significant (P value = less than 0.0001).Conclusions: Our results indicate that the HPV might contribute to the development of a subset of prostate malignant tumors and benign.