Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR

Vikrant Sharma,Deepak Kumar,Jaya Yadav,Sulochana Kaushik,Samander Kaushik,Divya Dhull

doi:10.9734/ibrr/2017/34463

Abstract

Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR

Highlights

Hepatitis C Virus (HCV) is one of the important viruses among other hepatitis viruses (A, B, D, and E) which are responsible for serious chronic infection of liver
The present study deals with the molecular detection of HCV with help of one-step reverse transcription polymerase chain reaction (RT-PCR) assay followed by nested PCR and agarose gel electrophoresis
Study Design: RNA extracted from the confirmed positive samples of HCV was utilized for the standardization of the one-step RT-PCR assay and nested PCR assay for diagnosis of HCV

Summary

Introduction

Hepatitis C Virus (HCV) is one of the important viruses among other hepatitis viruses (A, B, D, and E) which are responsible for serious chronic infection of liver. Another study estimated the number of HCV infected people may be 150–200 million globally [4,5,6]. Some recent study estimated that prevalence of HCV is about 1.5% in India [5]. HCV is mainly spread by direct contact with infected blood or body fluids. HCV infections are broadly divided into acute and chronic types. Chronic HCV infections are less prevalent but more risky than acute infections and can cause liver cirrhosis and cancer in some cases. HCV patients show symptoms like malaise, vomiting, fatigue, lethargy, anorexia, abdominal pain, myalgia, jaundice, mild hepatosplenomegaly, maculopapular rash and arthralgia [2,5,7]

Methods

Results

Discussion

Conclusion