Abstract
Aim:This study aimed to identify genes encoding resistance to tetracycline (TE) and plasmid-mediated resistance to quinolones in Escherichia coli isolates from clinical cases of avian colibacillosis in Sukabumi, Indonesia.Materials and Methods:A total of 25 E. coli archive isolates were collected in 2013-2017 from clinical cases of avian colibacillosis in Sukabumi, Indonesia. All isolates were tested for TE and quinolone resistance using the disk diffusion method. TE -resistant E. coli isolates were screened for the presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B), and qnr(S) genes were detected by multiplex PCR in quinolone-resistant E. coli isolates.Results:Result of this study shows that 19 of 25 (76%) E. coli isolates are resistant to oxytetracycline and 64% are resistant to TE; among them, 63.2% and 31.5% were positive tet(A) and tet(B), respectively. 13 out of 25 (52%) are resistant to ciprofloxacin and 36% are resistant to enrofloxacin either norfloxacin; among them, 61.6% were positive qnr(A), 7.7% were positive qnr(B), 23% were positive qnr(S), and 7.7% were positive both of qnr(A) and qnr(S).Conclusion:This study shows that a few pathogens of E. coli are resistant to TE and quinolone. The frequency of tet and qnr genes that are responsible for this resistance among avian pathogenic E. coli isolates in Sukabumi, Indonesia, was high.
Highlights
Avian colibacillosis is one of the most important infectious diseases in birds of all ages
The frequency of tet and qnr genes that are responsible for this resistance among avian pathogenic E. coli isolates in Sukabumi, Indonesia, was high
The result showed that avian pathogenic E. coli (APEC) isolates from clinical cases of avian colibacillosis in Sukabumi 76% (19 of 25 isolates) are resistant to oxytetracycline and 64% (16 out of 25 isolates) are resistant to TE
Summary
Avian colibacillosis is one of the most important infectious diseases in birds of all ages. This disease has an important economic impact on poultry production worldwide. Antibiotic as feed additives in an animal is required to reduce the economic consequences of bacterial disease, but it was contributed to the spread of antibiotic-resistant bacteria [1,2,3]. Antibiotic exposure is a source of stress that can create stress-induced resistance among bacteria [4]. TE has been improved and marketed under various trade names. They are used as growth promoters of farm animals worldwide. The resistance mechanisms for the TE class of antibiotics fall in four
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
