Abstract
Molecular Detection of Feline Herpesvirus by Means Polymerase Chain Reaction
Highlights
Feline herpes virus type 1 (FHV-1) was isolated in 1957 by Crandell and Maurer [1]
Based on the results obtained in this study, the following reflections can be made: in PCR2 all samples were positive, so it is thought that there was amplification when using primers 1 and 2 in PCR1, but because after PCR1 the samples and the positive control had a low number of amplicons, they did not generate a visible band in the UV transilluminator
The results of the PCR2 are explained by the condition of being a nested Polymerase Chain Reaction (PCR), where in the second reaction it was sought to amplify a segment immersed in the amplicon resulting from the first PCR, increasing the sensitivity and specificity of the test
Summary
Feline herpes virus type 1 (FHV-1) was isolated in 1957 by Crandell and Maurer [1]. It is a DNA virus linear double strand containing approximately 134 kilobases (kb), subdivided into long and short components of 104 and 30 kb respectively [2,3], which is immersed in a capsid of icosahedral symmetry constituted by 162 capsomeres. One serotype is known, which differs in virulence depending on the strain [7]. In adult cats it causes high morbidity and low mortality, whereas in small cats it can generate a mortality of 60% [8]
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