Abstract
Abstract Background: The efflux pump system is an important mechanism used by Pseudomonas aeruginosa to avoid the effects of antibiotics. Objectives: This research aimed to identify whether there was a relationship between the P. aeruginosa efflux pump genes and resistance to various antibiotics. Materials and Methods: The study was conducted at various hospitals in Babylon province from March 2022 to November 2022. One hundred thirty P. aeruginosa isolates from (burn, wound, urine, ear, and sputum) were recovered from patients. Both conventional methods and the Vitek2 system were used to identify these isolates. Antimicrobial susceptibility testing using disc diffusion was performed on the isolates. Efflux pump-specific primers were used in the polymerase chain reaction (PCR) technique for the molecular identification of MexAB-OprM genes. Results: Multidrug resistant (MDR) P. aeruginosa were present in 125 (96.2 %). The present results showed that mexA was present in all of the 130 isolates, mexb was found in 122 (93.84%) of isolates, and the Oprm gene was present in 127 (97.69%) of isolates. MexAB-OprM was significantly associated with MDR in this study (P < 0.05). Conclusion: The genes regulating efflux pumps are important as a key factor contributing to the development of MDR P. aeruginosa.
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