Molecular detection of dermatophytes isolated from tinea corporis infections in the Province of Wasit, Iraq

Abstract

Background: The detection of fungal components in clinical specimens using direct microscopy in conjunction with culture-based full identification is the basis for dermatophyte identification. Physiological, macro-morphological, and micro-morphological parameters are used to identify colonies phenotypically. In recent years, it has been shown that molecular methods are useful for classifying dermatophyte species. Methods: Fifty samples that were taken from human cases of tinea corporis were examined mycologically. Phenotypic techniques were used to identify isolated dermatophytes. Internal transcribed spacer (ITS) PCR amplification was used for molecular identification. Using a specific primer, PCR was utilized to analyze the 18S rRNA gene in fungal isolates and the genes for virulence factors. Results: Out of Fifty samples with tinea corporis infections identified. Among them, which 3species were identified distribution to 26 cases Trichophyton mentagrophytes with a percentage of 52%, was the most frequently isolated dermatophyte 12 cases Trichophyton rubrum with a percentage 24% and 12 cases Microsporum canis with a percentage 24%. PCR product analysis of the 18S rRNA gene in dermatophytes isolates showed that all dermatophytes ssp. 100% were positive for 18S rRNA gene they obtained dermatophyte isolates (n=50) as follows: 26 Trichophyton mentagrophytes 12 Trichophyton rubrum, 12 Microsporum canis.