Molecular detection of Cucumber green mottle mosaic virus variants from cucurbits fields in Iran

Abstract

Cucumber green mottle mosaic virus (CGMMV) is seed borne virus belonging to the genus Tobamovirus into family Virgaviridae, which transmit mechanically during agricultural practice, seed and through water. In this study, based on virus description cucurbits fields were observed to detect CGMMV. Therefore, biological purification was performed to recognise the type of symptoms incited by each isolates. Accordingly, local lesion host plants were inoculated with virus isolates. Following, the samples having CGMMV symptoms are selected to RNA extraction and molecular tests. Furthermore, to detect different strains of CGMMV, a pair of specific primers corresponding to full coat protein and a part of movement protein gene was applied to amplify a 523 bp fragment in reverse transcription polymerase chain reaction (RT-PCR). Eventually, an expected band with 523 bp length was revealed in nine isolates by RT-PCR. It is a first report of CGMMV from north-west of Iran by molecular methods.