Molecular detection of antibiotic resistance genes, and class I, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks

Abstract

This study aimed to investigate the molecular detection of antibiotic resistance genes, and class 1, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks.In this cross-sectional study, 650 samples were collected from one-day-old chicks from different regions of Iran and transferred to the Central Veterinary Laboratory-Iran Veterinary Organization (CVL-IVO). The prevalence of Integrons and resistance genes were determined by PCR. The pattern of antibiotic resistance was determined using Kirby-Bauer disk diffusion method. The data were analyzed using SPSS software through consensus table, and Chi-square test.Results showed the prevalence 33.3% of integrons. Frequency class I and II integrons were 26.6% and 6.6%, respectively. All isolates had GES, and gyrB genes, but 6.6% of those have DHAM gene. Other isolates did not show any resistance gene. The highest resistance was observed to Nalidixic acid, Penicillin and Nitrofurantoin (100%). The lowest resistance was reported to Trimethoprim with prevalence 6.6%.Our results showed a high prevalence of class Ι integrons, and a correlation between presence of class I and II integrons with resistance to Nalidixic acid, Penicillin, Nitrofurantoin, Colistin, Levofloxacin, and Ciprofloxacin in Salmonella enteritidis.