Abstract
Alfalfa witches’-broom (AWB) phytoplasma was detected in four leafhopper species ( Aceratagallia sp., Neokolla hieroglyphica, Cuerna septentrionalis and Macrosteles fascifrons) and infected alfalfa plant samples by PCR. The transmission results suggest that Neokolla and Cuerna species could be the vectors of AWB phytoplasma. The amplified DNAs yielded products of 1.5 kb (primer pair P1/P6), 196 bp (primer pair 1A/1B) and 1.2 kb (primer pair R16 F2/R2) by direct and nested-PCR assay. After RFLP analyses, using the four endonuclease enzymes, similar patterns were formed among the digested 1.2 kb PCR products of all the leafhopper and plant samples, indicating the presence of the same kind of a phytoplasma isolate. The RFLP analyses and nested-PCR assays, using the primer pair 1A/1B, revealed that this isolate was genetically related to the clover proliveration group
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