Abstract
Alfalfa witches’-broom (AWB) phytoplasma was detected in four leafhopper species ( Aceratagallia sp., Neokolla hieroglyphica, Cuerna septentrionalis and Macrosteles fascifrons) and infected alfalfa plant samples by PCR. The transmission results suggest that Neokolla and Cuerna species could be the vectors of AWB phytoplasma. The amplified DNAs yielded products of 1.5 kb (primer pair P1/P6), 196 bp (primer pair 1A/1B) and 1.2 kb (primer pair R16 F2/R2) by direct and nested-PCR assay. After RFLP analyses, using the four endonuclease enzymes, similar patterns were formed among the digested 1.2 kb PCR products of all the leafhopper and plant samples, indicating the presence of the same kind of a phytoplasma isolate. The RFLP analyses and nested-PCR assays, using the primer pair 1A/1B, revealed that this isolate was genetically related to the clover proliveration group
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.