Molecular Detection of abaI Genes in Acinetobacter baumannii Isolated from Clincal Speciemens in Some Iraqi Hospitals

Abstract

Acinetobacter baumannii is one of the most important causative bacteria of nosocomial infections. Attention turned toward this Gram negative bacterium due to its extensive resistance to antibiotics. Clinical One hundred thirty five samples sources (urine, sputum, wound burn,) were collected during the period from the between from the late September 2020 to the Mid-January 2021,from patients attending four hospitals in Anbar governorate which include (AlRamadi Genera Teaching ,Al- Fallujah Genera Teaching and Fallujah Maternity and Children’s Hospital). Regarding to the age group factor, the age group (40-49) years more susceptible to the infection is constituting 6(46.1%), followed by group(30-39) years with 3(23.1%) while (20-29)(50-59) and (60-69) years with percentage at 1(7.7)%. Also, the study indicated that the A.baumannii was disrupted equal in male (53.8%) and female (46.2 %) with appear no significant. All specimens were cultured on culture media including blood agar and MaCconkey agar. After the growth of bacteria, the isolates were identified by microscopic examination as well as the biochemical tests including the manual biochemical tests that include( oxidase, catalase, Simmon Citrate, Motility, Indole, Urease ,haemolysin,Lactose fermentationrowth at 44 ºC) .The identification of P. mirabilis confirmed by using the VITEK-2 system. A total of 13(9.6%) isolates of A.baumannii were identified,other bacteria obtained were identified as Escherichia coli, Pseudomonas aeruginosa, Klebsiella .pneumoniae and streptococcus spp. in percentage recorded (37%) , (22%) (20.2%) and (11%) respectively. The genomic DNA of A.baumannii isolates were extracted using wizard genomic DNA purification kit, the extracted genomic DNA was analyzed using 1% agarose gel electrophoresis, and then the concentration and purity of the extracted genomic DNA were determined using Nanodrop spectrophotometer device, to detect A.baumannii isolates by molecular methods, the extracted genomic DNA of these isolates was submitted for amplification to detect the specific gene Bap and abaI by the singleplex PCR assay. At the molecular level of this study, the results of PCR reaction showed the presence of abaI gene in 8 (61.54%) isolates carried the gene responsible for quorum sensing