Molecular detection gene 18s RNA putatif on Trichodina spp. which influence gourami larvae (Osphronemus gouramy) in Banyumas, Central Java

Abstract

Rokhmani, Ariyani E, Wahyono DJ. 2018. Molecular detection gen 18s RNA putatif on Trichodina spp. which influence gurami larvae (Osphronemus gouramy) in Banyumas, Central Java. Pros Sem Nas Masy Biodiv Indon 4: 35-38. Trichodina spp. are ectoparasitic pathogens of ciliata group. Infection of Trichodina spp. will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami in Bantul, Yogyakarta can reach 100%. Research has been conducted to determine which one Trichodina that infects the gouramy seeds of Village Beji, Kedungbanteng, Banyumas, Jawa Tengah following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (i) Sampling of gurami fish with purposive sampling which obtained from Village Beji, Kedungbanteng, Banyumas, (ii). Preparation of Trichodina spp. sample and its identification, and (iii). Molecular character obervation following detection of 18S rRNA gene. This study obtained that percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Village Beji, Kedungbanteng, Banyumas is 10%. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA T. paraheterodentata gene that infects gouramy fish in Banjarnegara, i.e. 45%.