Abstract
Background: Campylobacter spp. is recognized as one of the important bacterial agents of food borne diseases worldwide. C. jejuni and C. coli are the most frequently reported species causing bacterial gastrointestinal disorders in developed and some of the developing countries. Objectives: The goals of this survey were to detect and to differentiate C. jejuni and C. coli species in stool specimens and to determine the frequency of Campylobacter gastroenteritis in children referred to Bahonar hospital in Karaj city using polymerase chain reaction (PCR). Patients and Methods: One hundred sixty stool specimens were collected from neonates and children under 8 years old during August to October of 2009. PCR was optimized to amplify a 400 bp fragment of the cad F gene of Campylobacter genus in the clinical specimens, then multiplex PCR optimization was carried out using hipO and asp primers. C. jejuni RTCC 1097 and C. coli RTCC 1113 reference strains were used as positive controls and only the PCR positive specimens were examined by this method. Results: The results revealed the amplification of a 400 bp DNA fragment and Campylobacter contamination in 11.3 % of the specimens. Of 18 PCR positive specimens examined by duplex PCR method, 4 (22.2%) were identified as C. jejuni, 2 (11.1%) as C. coli, 3 (16.6%) as mixed infection with both species and 9 (50% of the positive specimens) were identified as non coli – non jejuni Campyltobacter. The sensitivity of the single PCR method at the DNA level was determined to be 100 pg and the specificity of the method was determined using 6 other important bacterial agents of gastrointestinal diseases. Conclusions: Although the findings of this survey confirmed the presence of C. jejuni and C. coli species in half of the positive specimens, the probable role of the other species of Campylobacter in children gastroenteritis was unexpected.
Highlights
Campylobacter spp. is recognized as one of the important bacterial agents of food borne diseases worldwide
Of 18 polymerase chain reaction (PCR) positive specimens evaluated by duplex PCR technique, 4 (22.2%) were detected as C. jejuni, 2 (11.1%) as C. coli, 3 (16.6%) as mixed infection with both species and 9 (50% of the positive samples) were detected as non coli – non jejuni Campylobacter species (Figure 2)
Campylobacter species, primarily C.jejuni and C. coli, are recognized as important bacterial agents of gastroenteritis in human [1, 3] and domestic animals especially poultry, livestock and companion animals [7]. The goals of this survey were to detect and to differentiate C. jejuni and C. coli species in stool specimens and to determine the frequency of Campylobacter gastroenteritis in children referred to Bahonar hospital in Karaj city using PCR
Summary
Campylobacter spp. is recognized as one of the important bacterial agents of food borne diseases worldwide. Objectives: The goals of this survey were to detect and to differentiate C. jejuni and C. coli species in stool specimens and to determine the frequency of Campylobacter gastroenteritis in children referred to Bahonar hospital in Karaj city using polymerase chain reaction (PCR). C. jejuni RTCC 1097 and C. coli RTCC 1113 reference strains were used as positive controls and only the PCR positive specimens were examined by this method. Conclusions: the findings of this survey confirmed the presence of C. jejuni and C. coli species in half of the positive specimens, the probable role of the other species of Campylobacter in children gastroenteritis was unexpected. The results of the limited published molecular surveys on Campylobacter infections in Iran confirmed the major role of jejuni and coli species in the cases of Campylobacteriosis [4]
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