Molecular detection and phylogenetic analysis of a Shiga toxin-producing strain Escherichia coli (partial rfbE and fliCh7 gene), serotype O157:H7 isolated from a living chicken of a traditional market in Indonesia

Abstract

The objective of this study was to identify a Shiga toxin-producing strain Escherichia coli partial rfbE and fliCh7 gene of O157:H7 isolated from a faeces sample collected from a live chicken in a traditional market in Bogor, Indonesia. The isolate was investigated using multiplex polymerase chain reaction (PCR) to detect stx1, stx2, rfbE, and fliCh7 gene of STEC O157:H7. Then, sequencing was applied to identify the antigen markers, which are the rfbE and fliCh7 genes. In the present study, the isolate which was obtained from a live chicken was successfully identified as STEC O157:H7 strain. This conclusion was based on multiplex PCR and a nucleotide sequence analysis. This pathogen was not only found in the live chicken, but it was further suggested that the rfbE and fliCh7 genes can be used as alternative targets for molecular identification of this pathogen.