Year-end Sale % OFF 
Abstract
BackgroundFirst identified in the United States in 2016, porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus exhibiting a wide range of clinical syndromes, which may be associated with the pathogenicity observed in pigs.ResultsThe aim of this study was to identify and characterize the full genome sequence of PCV3 strains circulating in Northeast China. Herein, 105 lung samples isolated from sick pigs in Northeast China during 2018 were analyzed for PCV3. Using PCR, the total PCV3-positive rate was 33.3% (35/105), with rates of 17.8% (8/45), 66.7% (10/15), and 37.8% (17/45) in Heilongjiang, Jilin, and Liaoning province, respectively. Additionally, our findings showed that PCV3-positive samples had a high rate of co-infection with PCV2, PPV6, and PPV7. To study the evolution of the PCV3 in Northeast China, we sequenced the entire genome of 13 strains of PCV3. The results of phylogenetic analyses revealed that PCV3 could be divided into two clades, PCV3a and PCV3b. Interestingly, a G deletion at position 1072 was found in the 1999 nt genome of PCV3-CN2018LN-4 (MH277118). The G deletion terminated replicase protein translation and induced a truncated replicase protein.ConclusionThese results contribute to the understanding of PCV3 molecular epidemiology and evolution in Northeast China. A new strain of PCV3 with truncated replicase protein was identified.
Highlights
First identified in the United States in 2016, porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus exhibiting a wide range of clinical syndromes, which may be associated with the pathogenicity observed in pigs
Similar to Porcine circovirus type 2 (PCV2), PCV3 has a circular single-stranded DNA genome ranging in size from 1999 to 2001 nucleotides, containing two major open reading frames (ORFs), ORF1 and ORF2, which code for a 296 amino acid replicase protein and 214 aa capsid protein, Ha et al BMC Veterinary Research (2018) 14:321 respectively [7, 8]
These data indicate that a high co-infection rate of PCV3 with PCV2, Porcine parvovirus 6 (PPV6), and Porcine parvovirus 7 (PPV7) exists, which provide valuable information for further study into the pathology of PCV3 in association with PCV2, PPV6, and PPV7
Summary
First identified in the United States in 2016, porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus exhibiting a wide range of clinical syndromes, which may be associated with the pathogenicity observed in pigs. Porcine circovirus 1 (PCV1) was initially identified in the 1970s as a contaminating agent in pig kidney cells and was considered nonpathogenic for swine [2, 3]. The study by Li et al demonstrated that PCV3 could be divided into two clades using the complete coding sequences [21, 22]. 62 complete coding sequences (13 in this study) were used for phylogenetic analysis and dividing PCV3 into different clades. A new strain of PCV3 with a 13 aa deletion in the replicase protein was identified
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
