Abstract
Rice plants showing orange leaf disease symptoms were observed in Southern Vietnam. A nested polymerase chain reaction assay using phytoplasma-universal primer pairs P1/P7 and R16F2n/R16R2 amplified products of approximately 1.2 kb only from symptomatic rice plants. Sequence analysis of 16Sr DNA amplicons showed that the phytoplasma strain VNTG-4 shared 100% sequence homology with phytoplasma strains from rice in Thailand, members of the 16SrI group. This is the first detection and identification of a phytoplasma associated with the orange leaf disease in Vietnam.
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