Abstract
Development of effective methods for cell separation is one of the major objects in the field of biomedical science.1 Separation of blood cells has become increasingly practical and more widely used in research and in the clinical field. Especially, there is widespread utility of separating B and T cells, the two major sub-populations of lymphocytes. In the clinical field, their separation is important in therapy and diagnosis of immunodiseases as well as in assessing the matching of donor-recipient pairs in transplantation. In the field of fundamental and applied biology, preparative separation of lymphocyte subpopulations is essential process for producing high-value biological substances, including interferon, lymphokine, and mono-clonal antibodies.
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