Abstract
BackgroundAegilops geniculata Roth is closely related to common wheat (Triticum aestivum L.) and is a valuable genetic resource for improvement of wheat.ResultsIn this study, the W19513 line was derived from the BC1F10 progeny of a cross between wheat ‘Chinese Spring’ and Ae. geniculata SY159. Cytological examination showed that W19513 contained 44 chromosomes. Twenty-two bivalents were formed at the first meiotic metaphase I in the pollen mother cellsand the chromosomes were evenly distributed to opposite poles at meiotic anaphase I. Genomic in situ hybridization demonstrated that W19513 carried a pair of alien chromosomes from the M genome. Fluorescence in situ hybridization confirmed detection of variation in chromosomes 4A and 6B. Functional molecular marker analysis using expressed sequence tag–sequence-tagged site and PCR-based landmark unique gene primers revealed that the alien gene belonged to the third homologous group. The marker analysis confirmed that the alien chromosome pair was 3Mg. In addition, to further explore the molecular marker specificity of chromosome 3Mg, based on the specific locus amplified fragment sequencing technique, molecular markers specific for W19513 were developed with efficiencies of up to 47.66%. The W19513 line was inoculated with the physiological race E09 of powdery mildew (Blumeria graminis f. sp. tritici) at the seedling stage and showed moderate resistance. Field inoculation with a mixture of the races CYR31, CYR32, CYR33, and CYR34 of the stripe rust fungus (Puccinia striiformis f. sp. triticii) revealed that the line W19513 showed strong resistance.ConclusionsThis study provides a foundation for use of the line W19513 in future genetic research and wheat improvement.
Highlights
Aegilops geniculata Roth is closely related to common wheat (Triticum aestivum L.) and is a valuable genetic resource for improvement of wheat
Cytological observations The W19513 line was derived from the cross between CS and SY159, followed by one backcross to CS and repeated selfing to generate the BC1F10 population
Two chromosomes showed the probe signal (Fig. 2A). This result implied that a pair of alien chromosomes were added to the genome of W19513
Summary
Aegilops geniculata Roth is closely related to common wheat (Triticum aestivum L.) and is a valuable genetic resource for improvement of wheat. Wheat (Triticum aestivum L.) is an important grain crop cultivated worldwide. Wang et al BMC Plant Biology (2021) 21:575 all represent severe challenges to food production [4]. Maintaining the sustainable production and stability of wheat grain yields is closely associated with China’s food security and socio-economic development strategies [7, 8]. Wild relatives of wheat (including wild and cultivated forms) are a reservoir for a large number of genes for desirable agronomic traits, and for example, are an important source of disease resistance genes for cultivated wheat [9]
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