Abstract
Orchidaceae is a highly evolved and largest angiosperm family, which includes enormous number of species and their hybrids. Recent molecular cytogenetic studies of orchid hybrids have successfully started to reveal their origin and chromosome evolution. Here, we constructed BAC libraries of the two orchid plants, Neofinetia falcata and Rhynchostylis coelestis, as molecular cytogenetic tools, which can be used for chromosome-based comparisons of specific regions between different species and their hybrids chromosomes. A total of 21,000 and 10,600 BAC clones with average insert sizes of 74.6 and 50.8 kb were obtained for the N. falcata and R. coelestis, respectively. Random BAC FISH analyses of the two orchid species revealed distribution of some repetitive sequences in these orchid chromosomes. Thus, these BAC clones are useful resources for understanding the genomic organization of the orchid plants.
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