Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust.

Zhi Li,Zhenglong Ren,Benju Yan,Tianheng Ren,Feiquan Tan,Shulan Fu,Zongxiang Tang

doi:10.1371/journal.pone.0163642

Abstract

Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.

Highlights

Common wheat (Triticum aestivum L.) is one of the most important crops in the world
Many alien genes have been transferred into bread wheat through chromosome translocations from its different relative genera, such as Secale cereale [5,6], Hordeum californicum [7], Leymus mollis [8], Agropyron elongatum [9], Haynaldia villosa [10], Thinopyrum [2,11], and Aegilops peregrina [12]
The present study reported 3 new primary 1RS.1BL translocation lines and a substitution line which were developed from the cross of the Chinese wheat cultivar Mianyang11 (MY11) and the Chinese local rye Aigan

Summary

Introduction

Common wheat (Triticum aestivum L.) is one of the most important crops in the world. The development of new wheat cultivars with higher yield and good resistance to diseases is the eternal goal of breeders. The alien substitutions or translocations of chromosomes between wheat and its relative species have played an important role in wheat improvement [1,2,3,4]. Many alien genes have been transferred into bread wheat through chromosome translocations from its different relative genera, such as Secale cereale [5,6], Hordeum californicum [7], Leymus mollis [8], Agropyron elongatum [9], Haynaldia villosa [10], Thinopyrum [2,11], and Aegilops peregrina [12].

Methods

Results

Conclusion