Abstract
Chromosomes of four Miscanthus (Andersson, 1855) species including M. sinensis (Andersson, 1855), M. floridulus (Schumann & Lauterb, 1901), M. sacchariflorus (Hackel, 1882) and M. lutarioriparius (Chen & Renvoize, 2005) were analyzed using sequentially combined PI and DAPI (CPD) staining and fluorescence in situ hybridization (FISH) with 45S rDNA probe. To elucidate the phylogenetic relationship among the four Miscanthus species, the homology of repetitive sequences among the four species was analyzed by comparative genomic in situ hybridization (cGISH). Subsequently four Miscanthus species were clustered based on the internal transcribed spacer (ITS) of 45S rDNA. Molecular cytogenetic karyotypes of the four Miscanthus species were established for the first time using chromosome measurements, fluorochrome bands and 45S rDNA FISH signals, which will provide a cytogenetic tool for the identification of these four species. All the four have the karyotype formula of Miscanthus species, which is 2n = 2x = 38 = 34m(2SAT) + 4sm, and one pair of 45S rDNA sites. The latter were shown as strong red bands by CPD staining. A non-rDNA CPD band emerged in M. floridulus and some blue DAPI bands appeared in M. sinensis and M. floridulus. The hybridization signals of M. floridulus genomic DNA to the chromosomes of M. sinensis and M. lutarioriparius genomic DNA to the chromosomes of M. sacchariflorus were stronger and more evenly distributed than other combinations. Molecular phylogenetic trees showed that M. sinensis and M. floridulus were closest relatives, and M. sacchariflorus and M. lutarioriparius were also closely related. These findings were consistent with the phylogenetic relationships inferred from the cGISH patterns.
Highlights
The genus Miscanthus (Andersson, 1855), belonging to the tribe Andropogoneae of family Poaceae, is a tall perennial grass with C4 photosynthesis (Stewart et al 2009)
Twenty-four Miscanthus accessions comprised of 6 M. sinensis, 6 M. floridulus, 6 M. sacchariflorus and 6 M. lutarioriparius were selected from different provinces of China and planted in the Miscanthus germplasm nursery located at the Hunan Agricultural University (Table 1)
M. floridulus has exhibited the large variation in chromosome length, whereas M. sinensis has displayed the large variation in centromeric index
Summary
The genus Miscanthus (Andersson, 1855), belonging to the tribe Andropogoneae of family Poaceae, is a tall perennial grass with C4 photosynthesis (Stewart et al 2009) It includes 14–20 species and has been considered as one of the most promising highyield fiber-based energy crops (Christian et al 2009, Brosse et al 2012). Four Miscanthus species, M. sinensis (Andersson, 1855), M. floridulus (Schumann & Lauterb, 1901), M. sacchariflorus (Hackel, 1882) and M. lutarioriparius (Chen & Renvoize, 2005), are most widely distributed. These have high biomass yield and are prone to interspecific hybridization, which lead to high genetic diversity (Liu et al 2013, Zhao et al 2017). Chromosome banding by Giemsa staining, fluorochrome staining or FISH with repetitive DNA sequences can provide additional characteristics to discriminate the chromosomes in the cell complement (Filion 1974, Sumner 1990, Koornneef et al 2003)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
