Abstract
Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races of stripe rust (CYR32 and CYR33). In this study, three disease resistance-related transcripts, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were acquired from among A. cristatum full-length transcripts. The BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655 were analyzed by using three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH). The results revealed that the introgression between A. cristatum P genome and wheat genome was observed in progenies of the genetic populations of the translocation line WAT655 and the physical positions of the three genes were considerably adjacent on A. cristatum 6PS (0.81–1.00) according to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 breeding lines selected from the progenies of the distant hybridization of wheat and A. cristatum, which showed resistance to physiological races of stripe rust (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, this study not only provides new insights into the introgression between A. cristatum P genome and wheat genome but also provides the desirable germplasms for breeding practice.
Highlights
Common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is one of the most cultivated cereal crops in the world
The results revealed that the fluorescence in situ hybridization (FISH) signals of the probes were located at the overlapping positions of A. cristatum 6PS (0.81–1.00) (Figure 5 and Figure S4), the three genes could be mapped to the considerably adjacent physical positions, which could constitute a potential disease resistance-related gene cluster on A. cristatum 6PS (0.81–1.00)
Wheat–A. cristatum 6P translocation lines WAT655, WAT638a, and WAT638b were produced by radiating hybrid plants of the wheat–A. cristatum 6P disomic addition line 4844-12, which were acquired via strict backcrossing with common wheat “Fukuho” as the recurrent parent and self-pollination
Summary
Common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is one of the most cultivated cereal crops in the world. The total global wheat acreage was approximately 220 million hectares, and the global yield was more than 770 million tons in 2020 [1]. 90% of cultivated wheat is susceptible to Pst, and more than five million tons of the wheat harvest are lost annually [4,5]. With the emergence of new Pst races and the variations in existing Pst races, many wheat varieties have lost resistance to stripe rust [6,7]. Providing new wheat germplasms with resistance to Pst infection is necessary for advancing wheat breeding
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