Abstract
Abstract: Few twinning events have been recorded in the West Indian manatee (Trichechus manatus, Sirenia: Trichechidae) and no previous published study has provided confirmation of this phenomenon based in molecular tools. Here we investigate a possible case of twinning in an endangered Brazilian population of T. manatus using molecular tools. We analyzed two male neonates found stranded in Ceará State, on the northeastern coast of Brazil. The DNA of both individuals was isolated, and 10 microsatellite loci were amplified and genotyped. Following the identification of the alleles, the probabilities of identity by descent (∆7 and ∆8) and relatedness (rxy) were calculated using estimators that evaluate inbreeding. The two individuals shared most of the alleles, with differences in the genotypes being identified in only two loci. All the estimators identified a level of relatedness compatible with that found between siblings (selfed or outbred), indicating they were dizygotic twins. This is the first confirmed case of fraternal twins in free-ranging West Indian manatees in South America. The recognition of this type of twinning provides elements to improve actions for the rehabilitation of stranded animals and their subsequent release to the environment.
Highlights
The West Indian manatee (Trichechus manatus Linnaeus, 1758) is an aquatic mammal which is considered vulnerable to extinction due to the natural processes and anthropogenic impacts that have affected its populations, primarily over the past two centuries (Deutsch et al 2008)
The unregulated human occupation of these environments (Borges et al 2007, Silva et al 2016) has been the principal factor leading to the high rate of neonate strandings on the northeastern coast of Brazil (Meirelles 2008, Balensiefer et al 2017)
We investigate a rare report of possible case of twinning in two neonate West Indian manatees found stranded in Ceará State, on the northeastern coast of Brazil, contributing to a broader understanding of this phenomenon and proposing the use of an applicable methodology for future similar reports
Summary
The West Indian manatee (Trichechus manatus Linnaeus, 1758) is an aquatic mammal which is considered vulnerable to extinction due to the natural processes and anthropogenic impacts that have affected its populations, primarily over the past two centuries (Deutsch et al 2008). Reports of twins in free-ranging West Indian manatees are scant, and monitoring data indicate that multiple births occur extremely infrequently (Marmontel 1995, Rathbun et al 1995).
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