Abstract
Erwiniacarotovora subsp. carotovora strain EC14 produces pectate lyases that elute in the void volume from DEAE cellulose DE53 columns. Two pBR322 derivatives containing shotgun-cloned EC14 DNA mediate the production of pectate lyases in Escherichia coli strain HB101 that elute in the void volume from these columns. The EC14 DNA insert in pDRl is 3.4 kilobases in length and contains no internal BamHI sites. Plasmid pDR30 contains an EC14 DNA insert which is 2.4 kilobases in length and is flanked by BamHI sites. There is no hybridization between the EC14 DNA inserts of these two plasmids. Therefore two separate EC14 chromosomal DNA fragments mediate the production of these pectate lyases. Evidence indicates the possibility that plasmid pDR30 also mediates the production of a polygalacturonase.
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