Abstract
Chloride channels in the ocular ciliary epithelium are believed to play a key role in aqueous humor formation. We isolated a cDNA clone from a λUni-ZAP cDNA library of human nonpigmented ciliary epithelial (NPE) cells encoding the swelling-induced chloride channel/channel regulator PI Cln. The human clone contains an open reading frame of 237 amino acids (M r 26,293). The deduced human amino-acid sequence shows 90.2% and 92.7% identity with counterparts isolated from rat kidney and the canine kidney epithelial cell line MDCK. Human NPE cell lines exhibited significant levels of pI Cln transcripts. Complementary perforated-patch, whole-cell patch clamping demonstrated that swelling activates Cl − channels of the NPE cells, as suggested by ruptured-patch measurements. The results document the molecular isolation and identification of a human cDNA clone of a Cl − conductance regulator from ocular cells displaying volume-activated Cl −channels.
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