Molecular cloning of LMO4 , a new human LIM domain gene

Abstract

We have identified a new human LIM domain gene by isolating an autoantigenic cDNA clone from a human breast tumor cDNA library. The predicted amino acid sequence of the cDNA clone’s 495 bp open reading frame contains two tandem LIM domain motifs, and within the LIM domain region there is 62% identity with the analogous region of the LIM-only gene LMO1. The homology to LMO1 is restricted to the 360 bp region encoding the tandemly repeated LIM domains, the rest of the open reading frame as well as the extensive, GC-rich 5′ untranslated region, and 3′ region of the 2 kb cDNA sequence are unrelated to any known genes. This gene has been designated LMO4.