Molecular cloning of insulin-like growth factor 3 (igf3) and its expression in the tissues of a female damselfish, Chrysiptera cyanea, in relation to seasonal and food-manipulated reproduction

Abstract

Food availability is a permissive determinant that drives gonadal activity in fish. The present study aimed to clarify the interactions between reproductive and nutritive statuses in the sapphire devil (Chrysiptera cyanea), a tropical damselfish with a long-day preference for reproduction. Insulin-like growth factor 3 (IGF3), a novel IGF that likely plays a role in gonadal maturation, was closely monitored in the sapphire devil. The cDNA of sapphire devil igf3 had an open reading frame of 443 base pairs (146 amino acid residues). Phylogenetic analyses revealed that sapphire devil IGF3 was clustered within the teleost IGF3 family. The transcript levels of sapphire devil igf3 increased in the brain, liver, and ovary of the fish during the late vitellogenic phase, suggesting that it plays a role in reproduction. Immersion of the fish in seawater containing estradiol-17β suppressed transcript levels of sapphire devil igf3 in the liver, but not in the brain, suggesting that intensive protein synthesis in relation to vitellogenesis negatively impacts somatic metabolism in this tissue. When fish were reared with high or low food under conditions of photoperiod (LD = 14:10) and temperature (at 25–28 °C) during the non-reproductive season, ovarian development was induced in high-food fish. Furthermore, prior to ovarian development in the high-food fish, the transcript levels of sapphire devil igf3 increased in the brain, liver, and ovary. These results indicated crosstalk between the reproductive and growth networks and suggested that a metabolic shift, from growth mode to reproductive mode, occurs in peripheral tissues when nutritive status is improved under suitable conditions of photoperiod and water temperature.