Molecular Cloning of Hepatic Insulin-like Growth Factor-1 cDNA and Sequence Analysis in Lizards

Abstract

Event Abstract Back to Event Molecular Cloning of Hepatic Insulin-like Growth Factor-1 cDNA and Sequence Analysis in Lizards Christine A. Duncan1, 2* and Henry B. John-Alder3 1 California State University, Fresno, Department of Biology, United States 2 Rutgers, The State University of New Jersey, Graduate Program in Endocrinology and Animal Biosciences, United States 3 Rutgers, The State University of New Jersey, Department of Ecology, Evolution, and Natural Resources, United States The insulin-like growth factor (IGF) hormone family is well conserved in structure and function across all vertebrates. This high degree of conservation is likely due to the critical roles that these hormones play in development, growth, and metabolism. To date, the structure and function of insulin-like growth factors have been described in a wide range of vertebrate taxonomic groups, however, lizards have received little attention. Therefore, the objective of this study was to indentify IGF-1 cDNA sequence data from five lizard species. Primers designed against conserved regions of IGF-1 were used to generate cDNA from Sceloporus undulatus, S. jarrovii, Anolis sagrei, Coleonyx elegans, and Goniurosaurus lichtenfelderi. The cDNA was cloned into the pCR™4-TOPO® TA vector and sequenced. The resulting cDNA base sequences were converted to amino acid (AA) sequences. Sceloporus undulatus IGF-1 contained 55 AA and S. jarrovii, A. sagrei, C. elegans, and G. lichtenfelderi IGF-1 contained 43 AA. Comparison of the deduced AA sequences of IGF-1 to human IGF-1 confirms high sequence identity: S. undulatus (80%), S. jarrovii (74%), A. sagrei (72%), C. elegans (74%), and G. lichtenfelderi (77%). High IGF-1 sequence identity was observed between these species of lizards (86-100%). Although these species belong to the same order, variation occurred in the C domain, which is likely due to the fact that Iguania and Gekkota diverged approximately 240 million years ago. The percent of sequence identity is associated with the degree of relatedness between species and progressively decreases with evolutionary distance. To our knowledge, we are the first to clone IGF-1 from any species of reptile. The partial IGF-1 sequences cloned from lizards contribute to the growing body of literature that indicates that the high percent of sequence identity is associated with the essential involvement of IGF-1 in growth regulatory mechanisms among vertebrates. Acknowledgements Supported by the Society for Integrative and Comparative Biology, Graduate School-New Brunswick, and School of Environmental and Biological Sciences. Keywords: cloning, IGF, IGF-1, Lizard, Sequencing Conference: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011. Presentation Type: Poster Topic: Growth and aging Citation: Duncan CA and John-Alder HB (2011). Molecular Cloning of Hepatic Insulin-like Growth Factor-1 cDNA and Sequence Analysis in Lizards. Front. Endocrinol. Conference Abstract: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology. doi: 10.3389/conf.fendo.2011.04.00130 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Dr. Christine A Duncan, California State University, Fresno, Department of Biology, Fresno, CA, 93740, United States, cduncan@csufresno.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Christine A Duncan Henry B John-Alder Google Christine A Duncan Henry B John-Alder Google Scholar Christine A Duncan Henry B John-Alder PubMed Christine A Duncan Henry B John-Alder Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.