Molecular cloning of glpFKRD of a mushroom-pathogenic bacterium, Pseudomonas tolaasii strain PT814: induction of an avirulent mutant carrying a single mini-Tn5km 1 insertion in glpD

Abstract

Pseudomonas tolaasii strain PT 814 causes brown blotch disease in cultivated mushrooms. A pleiotropic avirulent mutant was isolated by mini-Tn5km 1 insertion mutagenesis. The insertion was localized in an open reading frame (ORF) predicted to encodesn-glycerol-3-phosphate dehydrogenase (glpD). ORFs that should encode its regulator, kinase, and facilitator were also identified as theglp gene cluster in the bacterium. The data suggest that theglp system may contribute to the ecology of this pathogen.