Abstract
Abstract A gene responsible for the production of iturin and surfactin was cloned and analyzed in the original wild strain Bacillus subtilis RB14, a coproducer of surfactin and iturin, as well as in B. subtilis MI113, a derivative of strain B. subtilis 168. The cloned gene lpa (lipopeptide antibiotic production) permitted only surfactin production in strain MI113, but when it was destroyed in RB14, the defective strain simultaneously lost the ability to produce both surfactin and iturin. The ability was restored by the introduction of the intact lpa gene into the strain.
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