Abstract
Nine partial sequences characteristic of cytochrome P450 (CYP) were obtained by RT-PCR using degenerate primers designed from conserved regions of the CYP6 family. The sequences were CYP6A1, CYP6D1 and seven new CYPs. Full amino acid sequences of CYP6D1 and two new CYP isoforms, CYP6A24 and CYP6D3v2 were analyzed. The amino acid sequence of CYP6D1 in the pyrethroid resistant strains was identical with that of a susceptible strain, CS, already reported. Northern blot analysis indicated that CYP6D1, CYP6A24 and CYP6D3v2 were overexpressed in all pyrethroid resistant strains when compared with the susceptible strain. In conclusion, pyrethroid resistant strains in Japan show simultaneous overexpression of several CYP isoforms.
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