Molecular cloning, mRNA expression, and characterization of calm×odulin genes in hybrids of Acanthopagrus schlegeli (♀)× Pagrosomus major ( ♂ )

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 黑鲷♀×真鲷♂反交子代与黑鲷的CaM基因克隆与mRNA表达分析 DOI: 作者: 作者单位: 1. 江苏省海洋水产研究所, 江苏省海水鱼类遗传育种重点实验室, 江苏 南通 226007;2. 南京师范大学 生命科学学院 生物多样性与生物技术重点实验室, 江苏 南京 210023 作者简介: 陈淑吟(1966-),女,博士,研究员,研究方向为鱼类种质资源与遗传育种.E-mail:shuyinchen89@163.com;李鹏(1979-),并列第一作者,男,博士,副教授,研究方向为动物比较基因组与分子进化.E-mail:biolipeng@163.com 通讯作者: 中图分类号: Q96 基金项目: 江苏省海洋与渔业局水产三新工程重大项目（D2015-17）；南通市科技局应用基础研究项目（MS12015071，MS12015070）. Molecular cloning, mRNA expression, and characterization of calm×odulin genes in hybrids of Acanthopagrus schlegeli (♀)×Pagrosomus major (♂) Author: Affiliation: 1. Institute of Oceanology & Marine Fisheries, Nantong 226007, China;2. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为分析黑鲷♀×真鲷）反交子代在生长、发育等经济性状优于黑鲷亲本（PACaM与在仔鱼及2龄成鱼不同组织的表达特征。研究结果表明，基因cDNA全长1241 bp，均具有一个450 bp的开放阅读框，编码149个氨基酸，分子量约16.84 kD，等电点为4.09；序列比对、结构比较等分析表明，在两种鱼的仔鱼及成鱼的脑与性腺中有较高表达；<0.05），在脑、肝及肾中的表达没有明显差异（基因在生长与繁殖中的重要作用。研究结果为鲷科属间杂交获得的子代与亲本性状差异的功能基因表达提供一些基础资料。 Abstract:The hybrid offspring from black porgy () have been successfully farmed in an attempt to develop new marine aquaculture species with optimal target traits. To analyze the molecular mechanism of different growth traits between parent fish (As) and hybrids (PA), the full-length cDNA sequences of ) from each were cloned and identified. The expression profiles of genes in six different tissues of two-year-old and 30 d larvae of F1 generation hybrids and pure individuals were evaluated and compared using real-time quantitative PCR. The gene is 1241 bp, both including an open reading frame (ORF) of 450 bp and encoding a peptide of 149 amino acids with an estimated molecular mass of 16.84 kD and a predicted isoelectric point (pI) of 4.09. Sequence alignment, structure comparison, and bioinformatics analyses showed that family. Functional domain analysis indicated that the protein has four EF-h and calcium-binding domains, with sixteen Ca2+ binding sites. are non-secretory and non-transmembrane proteins, and likely have their functional location in the cytoplasm. Quantification of expressed mRNA showed comparatively high levels in the brain and gonadal tissues of two-year-old fish and in larval fish. in gill, muscle, and gonadal tissue, as well as in larvae, but not significantly different in the brain, liver, or kidney tissues ( transcripts in the hybrid larvae, and the highest enrichment of in growth and development. This study provides a basis for further research into gene function and differing genetic traits between the parent and hybrid offspring of genus crosses of Sparidae. 参考文献 相似文献 引证文献